Technology

General Toolbox

The independent myBIOS Pichia pastoris expression toolbox is built on >15 years of experience in Pichia pastoris strain development. The core of the platform is our methanol-free expression technology which combines promoter, strain and process developments.

To maximize productivity the expression strategy must be tailored to the protein of interest. We established a broad expression toolbox (host strains, secretion signals, fusion proteins, chaperones, etc.) and high-throughput analytics for cost and time efficient strain optimization. In addition, we perform tailor made custom knock-outs and create co-expression strains to further increase protein titres or modify protein modifications.

The myBIOS expression toolbox is also designed to fulfil quality, safety and regulatory requirements. For example, strains can be generated without antibiotic resistance markers.

Various promoters

  • Methanol induced
  • Methanol free (P1 dual): induction with methanol or alternative inducer
  • Methanol free (P2 tight): alternative inducer, no leaky expression

Markers

  • Antibiotic
  • Antibiotic recycled (removed from genome)
  • Auxotrophic

Host strain collection

  • Wild type strains: CBS2612, CBS7435
  • Phenotype strains
  • Auxotrophic strains
  • Protease deficient strains

Customized knock out strains

Secretion signals

Chaperone library

Fusion proteins

Technology

Pichia
pastoris Methanol-free
platform General
Toolbox